These are not the physics vectors.

Biology Level pending

A vector is 1 kb long. Cloning is done, and a 1 kb insert is ligated into the vector, for a total of 2 kb, theoretically if the ligation worked. The ligation was left overnight at \(4^{\circ} C\) and in the morning, was electroporated into bacteria. Antibacterial selection was performed, and the antibacterial resistance gene is found in the vector. When the bacteria grew up, miniprep was done to obtain the plasmids.

We can check if the cloning was done correctly using restriction digestion, and then running the digestion products through an EtBr agarose gel.

Which combination of restriction enzymes uses the minimum amount of restriction enzymes but will tell us whether the cloning worked?

Assume that there are no dead restriction sites, and assume that you had the choice of the restriction sites to affix to the inserts, and assume that all the possible restriction sites you could have ever wanted in the vector could be there, if you want them to be there. Also assume that if I say 2 or 3 restriction enzymes, they are all different enzymes, for different restriction sites. One specific restriction enzyme works on only one specific restriction site.


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